Affiniteit koppelingsmedium, CNBr-geactiveerde Sefarose™ 4B

Leverancier: Cytiva
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Sepharose™
17-0430-02EA 4045 EUR
17-0430-02 17-0430-01
Affiniteit koppelingsmedium, CNBr-geactiveerde Sefarose™ 4B
Eiwitzuivering Protein Purification Resins
CNBr-geactiveerde Sefarose™ 4B is een vooraf geactiveerd medium dat gebruikt wordt voor het koppelen van antilichamen of andere grote -NH₂ groepen bevattende proteïnen aan het Sepharose™ medium, door de cyanogeenbromide methode, zonder een tussenliggende spacer arm.

  • Multipoint attachment resulting in a chemically stable product
  • Over 25 years proven track record for coupling ligands containing primary amino groups
  • The coupling reaction by the cyanogen bromide method is spontaneous, rapid and easy to carry out
  • Stable to all commonly used aqueous solutions
  • Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride
  • Stable in organic solvents, such as 50% dimethylformamide and 50% dioxane

It provides a very convenient way to immobilise ligands by the cyanogen bromide method. The coupling reaction is spontaneous, rapid and easy to carry out. The application area covers immobilisation of proteins, peptides and nucleic acids. For immobilising antibodies or other large proteins containing -NH₂ groups by coupling them to the matrix.
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