About this item
Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry or microscopy.
- Highly dead-cell specific
- Covalent labelling with reactive dyes
- Withstand fixation and permeabilisation
- The most available colours
- Dye options designed for spectral flow
Live-or-Dye™ Fixable Viability Stains are cell membrane impermeable amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. On live cells, the dyes react with surface proteins, but these are much less abundant than intracellular proteins, resulting in low staining levels compared to dead cells. Live-or-Dye™ labelling is extremely stable, allowing the cells to be fixed and permeabilised without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimised to maximise live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.
Live-or-Dye™ Fixable Viability Stains are cell membrane impermeable amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. On live cells, the dyes react with surface proteins, but these are much less abundant than intracellular proteins, resulting in low staining levels compared to dead cells. Live-or-Dye™ labelling is extremely stable, allowing the cells to be fixed and permeabilised without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimised to maximise live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.
The dyes are named for their excitation/emission wavelengths. Choose from a wide variety of colours with fluorescence from UV to near-IR. Live-or-Dye™ 488/515, 568/583, 594/614, & 640/662 can be used for either flow cytometry or fluorescence microscopy. Live-or-Dye™ 510/550 & 665/685 have been validated for use in spectral flow cytometry on the Cytek® Aurora. Live-or-Dye™ 787/808 offers the best performance for the 808 laser line, effectively freeing up the channels in the visible spectrum.
Kit Components:
Live-or-Dye™ Fixable Viability Dye
Anhydrous DMSO
Product Information
- Catalog #Supplier #Laser linePkAvailabilityPrice
- Live-or-Dye™ 350/448, DAPI or violet emission filter, for flow cytometry, (λEx/λEm) 347/448 nmLive-or-Dye™ 350/448, DAPI or violet emission filter, for flow cytometry, (λEx/λEm) 347/448 nmBTIU32002-T32002-T355 nm50 TestsLog in for availability1 * 1 EA117,00 €
- Live-or-Dye™ 350/448, DAPI or violet emission filter, for flow cytometry, (λEx/λEm) 347/448 nmLive-or-Dye™ 350/448, DAPI or violet emission filter, for flow cytometry, (λEx/λEm) 347/448 nmBTIU3200232002355 nm200 TestsLog in for availability1 * 1 EA385,00 €
- Live-or-Dye™ 405/452, pacific blue emission filter, for flow cytometry, (λEx/λEm) 408/452 nmLive-or-Dye™ 405/452, pacific blue emission filter, for flow cytometry, (λEx/λEm) 408/452 nmBTIU32003-T32003-T405 nm50 TestsLog in for availability1 * 1 EA117,00 €
- Live-or-Dye™ 405/452, pacific blue emission filter, for flow cytometry, (λEx/λEm) 408/452 nmLive-or-Dye™ 405/452, pacific blue emission filter, for flow cytometry, (λEx/λEm) 408/452 nmBTIU3200332003405 nm200 TestsLog in for availability1 * 1 EA385,00 €
- Live-or-Dye™ 488/515, FITC emission filter, for flow cytometry and microscopy, (λEx/λEm) 490/515 nmLive-or-Dye™ 488/515, FITC emission filter, for flow cytometry and microscopy, (λEx/λEm) 490/515 nmBTIU32004-T32004-T488 nm50 TestsLog in for availability1 * 1 EA117,00 €
- Live-or-Dye™ 488/515, FITC emission filter, for flow cytometry, (λEx/λEm) 490/515 nmLive-or-Dye™ 488/515, FITC emission filter, for flow cytometry, (λEx/λEm) 490/515 nmBTIU3200432004488 nm200 TestsLog in for availability1 * 1 EA385,00 €
- Live-or-Dye™ 568/583, PE emission filter, for flow cytometry and microscopy, (λEx/λEm) 562/583 nmLive-or-Dye™ 568/583, PE emission filter, for flow cytometry and microscopy, (λEx/λEm) 562/583 nmBTIU32005-T32005-T488 or 561 nm50 TestsLog in for availability1 * 1 EA117,00 €
- Live-or-Dye™ 568/583, PE emission filter, for flow cytometry and microscopy, (λEx/λEm) 562/583 nmLive-or-Dye™ 568/583, PE emission filter, for flow cytometry and microscopy, (λEx/λEm) 562/583 nmBTIU3200532005488 or 561 nm200 TestsLog in for availability1 * 1 EA385,00 €
- Live-or-Dye™ 594/614, PE-Texas red emission filter, for flow cytometry and microscopy, (λEx/λEm) 561/624 nmLive-or-Dye™ 594/614, PE-Texas red emission filter, for flow cytometry and microscopy, (λEx/λEm) 561/624 nmBTIU32006-T32006-T488 or 561 nm50 TestsLog in for availability1 * 1 EA117,00 €
- Live-or-Dye™ 594/614, PE-Texas red emission filter, for flow cytometry and microscopy, (λEx/λEm) 561/624 nmLive-or-Dye™ 594/614, PE-Texas red emission filter, for flow cytometry and microscopy, (λEx/λEm) 561/624 nmBTIU3200632006488 or 561 nm200 TestsLog in for availability1 * 1 EA385,00 €
- Live-or-Dye™ 640/662, APC emission filter, for flow cytometry and microscopy, (λEx/λEm) 642/662 nmLive-or-Dye™ 640/662, APC emission filter, for flow cytometry and microscopy, (λEx/λEm) 642/662 nmBTIU32007-T32007-T633 or 640 nm50 TestsLog in for availability1 * 1 EA117,00 €
- Live-or-Dye™ 640/662, APC emission filter, for flow cytometry and microscopy, (λEx/λEm) 642/662 nmLive-or-Dye™ 640/662, APC emission filter, for flow cytometry and microscopy, (λEx/λEm) 642/662 nmBTIU3200732007633 or 640 nm200 TestsLog in for availability1 * 1 EA385,00 €
- Live-or-Dye™ 750/777, APC-Cy7 emission filter, for flow cytometry, (λEx/λEm) 755/777 nmLive-or-Dye™ 750/777, APC-Cy7 emission filter, for flow cytometry, (λEx/λEm) 755/777 nmBTIU32008-T32008-T633 or 640 nm50 TestsLog in for availability1 * 1 EA117,00 €
- Live-or-Dye™ 750/777, APC-Cy7 emission filter, for flow cytometry, (λEx/λEm) 755/777 nmLive-or-Dye™ 750/777, APC-Cy7 emission filter, for flow cytometry, (λEx/λEm) 755/777 nmBTIU3200832008633 or 640 nm200 TestsLog in for availability1 * 1 EA385,00 €
- Live-or-Dye™ 405/545, AmCyan emission filter, for flow cytometry, (λEx/λEm) 395/545 nmLive-or-Dye™ 405/545, AmCyan emission filter, for flow cytometry, (λEx/λEm) 395/545 nmBTIU32009-T32009-T405 nm50 TestsLog in for availability1 * 1 EA117,00 €
- Live-or-Dye™ 405/545, AmCyan emission filter, for flow cytometry, (λEx/λEm) 395/545 nmLive-or-Dye™ 405/545, AmCyan emission filter, for flow cytometry, (λEx/λEm) 395/545 nmBTIU3200932009405 nm200 TestsLog in for availability1 * 1 EA385,00 €
- Live-or-Dye™ 510/550, for spectral cytometryLive-or-Dye™ 510/550, for spectral cytometry32012.32012488 or 532 nm200 TestsLog in for availability1 * 1 EA385,00 €