Fast Library Quantification kits (for Q), sparQ

Leverancier: Quantabio
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QUNT95197-500EA 0 EUR
QUNT95197-500
Fast Library Quantification kits (for Q), sparQ
Accurate qPCR-based library quantification in 40 minutes.

  • Faster time to results – 50% shorter run time than traditional cycling protocols
  • Accurate, reliable quantification of NGS libraries of various sizes and GC-content
  • High amplification efficiency across a wide linear dynamic range
  • Stabilised, ready-to-use sparQ Fast Mastermix to reduce pipetting steps
  • Superior run to run uniformity ensuring highly precise measurements
  • qPCR is the most accurate method for library quantification

sparQ Fast Library Quant Kit provides rapid and accurate quantification of libraries prepared for sequencing on Illumina® NGS platforms. Accurate quantification of the number of amplifiable library molecules prior to loading onto a flow cell is a critical step in the NGS workflow and it ensures optimal cluster generation and cost-effective use of sequencing capacity. The sparQ Fast Library Quant Kit uses real-time quantitative PCR (qPCR) to specifically quantify the number of library molecules that possess the appropriate adapter tag at each end.

Accurate library quantification in 40 minutes: This kit is optimised for the Q qPCR instrument which uses a magnetic induction technology to rapidly heat samples coupled with fan forced air for cooling to acquire data more rapidly. The combination of the sparQ fast library quant kit and the Q instrument enables fast cycling, reducing qPCR run time by 50% compared to traditional cycling protocols.

Complete library quantification solution with unmatched convenience: sparQ Fast Library Quant Kit contains six stabilised, pre-diluted DNA standards, ready-to-use 1.25x mastermix pre-mixed with primer sets containing Illumina® P5 and P7 sequences, and an optimised buffer for diluting NGS library samples. This unique formulation minimizes pipetting steps and ensures precise qPCR results.

qPCR as the most accurate method for library quantification:Real-time quantitative PCR is the most sensitive and precise method for quantifying adapter-ligated DNA molecules. Other methods based on spectrophotometry, fluorometry, or microfluidic electrophoresis (e.g. nanodrop, Qubit, or Bioanalyzer) are acceptable for estimating the appropriate dilutions to use for library quantification. These methods, however, are prone to variabilities and inaccuracies due to factors such as sensitivity to contaminants or measurement of unsequenceable fragments. The qPCR-based sparQ Fast Library Quant Kit measures only library DNA fragments containing the appropriate adapter sequences on both ends and thus serves as the most accurate method for library quantification, which in turn facilitates optimal loading onto sequencing flow cells.
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