About this item
Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.
- Increase sensitivity by up to 100-fold
- Use lower primary antibody concentrations
- Boost signal in challenging samples, like human FFPE tissues
- Choose from six bright and photostable CF® dyes or biotin
Tyramide amplification, sometimes called Catalysed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labelling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to conventional avidin-biotin detection. Multiple rounds of tyramide signal amplification can be performed for multiplex detection.
The HRP goat anti-rabbit antibody provided in the kits has been adsorbed against human, mouse, and rat serum to minimise cross-reactivity. The kits include choice of six CF® dye tyramides or biotin tyramide. CF® dyes are line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes.
Product Information
- Catalog #Supplier #PkAvailabilityPrice
- Tyramide amplification kit with HRP goat anti-rabbit and CF®488A tyramide, Ex/Em - 490/515 nmTyramide amplification kit with HRP goat anti-rabbit and CF®488A tyramide, Ex/Em - 490/515 nm33001.330011 KITLog in for availability1 * 1 Kit842,00 €Supplier Article Number: 33001Local Article Number: BTIU33001
- Tyramide amplification kit with HRP goat anti-rabbit and CF®543 tyramide, Ex/Em - 541/560 nmTyramide amplification kit with HRP goat anti-rabbit and CF®543 tyramide, Ex/Em - 541/560 nm33004.330041 KITLog in for availability1 * 1 Kit842,00 €Supplier Article Number: 33004Local Article Number: BTIU33004
- Tyramide amplification kit with HRP goat anti-rabbit and CF®568 tyramide, Ex/Em - 562/583 nmTyramide amplification kit with HRP goat anti-rabbit and CF®568 tyramide, Ex/Em - 562/583 nm33007.330071 KITLog in for availability1 * 1 Kit842,00 €Supplier Article Number: 33007Local Article Number: BTIU33007
- Tyramide amplification kit with HRP goat anti-rabbit and CF®594 tyramide, Ex/Em - 593/613 nmTyramide amplification kit with HRP goat anti-rabbit and CF®594 tyramide, Ex/Em - 593/613 nm33010.330101 KITLog in for availability1 * 1 Kit842,00 €Supplier Article Number: 33010Local Article Number: BTIU33010
- Tyramide amplification kit with HRP goat anti-rabbit and CF®640R tyramide, Ex/Em - 642/662 nmTyramide amplification kit with HRP goat anti-rabbit and CF®640R tyramide, Ex/Em - 642/662 nm33013.330131 KITLog in for availability1 * 1 Kit842,00 €Supplier Article Number: 33013Local Article Number: BTIU33013
- Tyramide amplification kit with HRP goat anti-rabbit and CF®680R tyramide, Ex/Em - 680/701 nmTyramide amplification kit with HRP goat anti-rabbit and CF®680R tyramide, Ex/Em - 680/701 nm33016.330161 KITLog in for availability1 * 1 Kit842,00 €Supplier Article Number: 33016Local Article Number: BTIU33016
- Tyramide amplification kit with HRP goat anti-rabbit and Biotin-XX tyramideTyramide amplification kit with HRP goat anti-rabbit and Biotin-XX tyramide33019.330191 KITLog in for availability1 * 1 Kit842,00 €Supplier Article Number: 33019Local Article Number: BTIU33019